Fusion index myoblast
WebApr 1, 2024 · (B) The fusion index of differentiated immortal myoblast cultures was determined by the percentage of the number of nuclei in myotubes (>2 myonuclei) to the total number of nuclei in desmin-positive cells (n=3 for each condition, >1000 nuclei counted per experiment). (C) Nuclear RNA aggregates of expanded-CUG or -CCUG repeats in … Webwww.ncbi.nlm.nih.gov
Fusion index myoblast
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WebApr 30, 2014 · To determine the potential role of Stac3 in myoblast differentiation, C2C12 myoblasts transfected with Stac3 siRNAs or scrambled siRNA were induced to … WebFig. 3A shows the fusion index of the C2C12 cells on hybrid patterns. The results show a progressive decrease in myotube differentiation starting with the hybrid 301 followed by hybrid 601 and ...
WebMar 16, 2024 · In addition, IGF-1 supplementation could alleviate impaired myoblast differentiation caused by mitophagy deficiency, as evidenced by increased fusion index and myosin heavy chain expression. These findings provide new insights into the role of IGF-1 signaling and suggest that IGF-1 signaling can serve as a target for the research and ... WebFeb 1, 2024 · Indeed, C2C12 cells co-cultured with MC-SeC showed a fusion index of only 7.4±4.8% in comparison with the 68.8±3.4% fusion index of E-MC-treated myoblasts …
WebMay 26, 2016 · The differentiation index (DI) was calculated as the percentage of desmin-positive cells in relation to the total number of nuclei. In order to quantitatively assess the extent of myoblast fusion, a myogenic fusion index (MFI) was determined as the average number of myogenic nuclei present in multinucleated myotubes. WebNov 18, 2024 · In contrast, in C2C12 cells overexpressing Nox4, the fusion index was increased, whereas the differentiation index and MHC and myogenin protein expression were not affected compared to control. Interestingly, the expression of myomaker (Tmem8c), a fusogenic protein that controls myoblast fusion, was reduced in Nox4-knockdown …
WebFeb 14, 2024 · ICC allows for quantification of the fusion index, which is defined as the percentage of myoblast nuclei that have fused into fibers in a single time point . Fusion indices are the main tool for assessment of the differentiation potential of myoblasts . Newer live cell imaging tools allow assessment of myoblast differentiation in real-time.
WebJun 10, 2024 · The fusion index is commonly used in muscle cell culture assays to determine the amount of myoblast fusion [6,7,8,9]. To this end, nuclei are visualized using DNA binding compounds like Hoechst, and myotubes are stained using fluorescent labelled antibodies for structural muscle protein, mainly myosin heavy chain (MyHC) among others. griffith hubbardWebOct 19, 2024 · The fusion index of wild-type cultures was ∼58% after 7 days of differentiation, with an average of 6.9±2.1 nuclei per myotube (mean±s.d.; ... Taken together, our findings indicate that the regulation of myoblast fusion is more complex than previously anticipated and may involve both CDC50A-dependent (including different … griffith hsu entWebDownload scientific diagram Comparison of myotube density and fusion index after C2C12 treatment. C2C12 cells were differentiated for 4 days in the presence of 10 μM prostaglandin D2 (PGD2), 10 ... fifa online ticketWebMay 1, 2010 · Cell-cell fusion is a crucial and highly regulated event in the genesis of both form and function of many tissues. One particular type of cell fusion, myoblast fusion, is a key cellular process that shapes the formation and repair of muscle. Despite its importance for human health, the mechanisms un … griffith hsuWebAug 31, 2024 · Compared to WT, SOD1-mutant myoblasts had a significantly lower fusion index (p < 0.001) (Fig. 2F). Additionally, the number of myotubes per area in each culture was quantified as another measure ... griffith hsu mdWebDec 9, 2014 · The inhibition of SIRT3 expression mediated by the stable integration of SIRT3 short inhibitory RNA (SIRT3shRNA) in C2C12 myoblasts, resulted in: 1) abrogation of terminal differentiation - as evidenced by a marked decrease in the myoblast fusion index and a significant reduction of Myogenin, MyoD, Sirtuin 1 and Troponin T protein … fifa online ticketingWebAug 9, 2024 · To validate this assay, myoblast fusion index measurements and immunohistochemistry (see below) were performed in parallel. Fig. 1.Decreased myoblast fusion and myotube formation in cerebral palsy (CP). A: example of a normalized differentiation assay performed over ~42 h with the iCELLigence RTCA system. Green … griffith hughes