Chondrocyte alcian blue staining
WebNov 1, 2000 · Following 21 days in culture with chondrogenic differentiation media, the micromass pellets were sectioned and stained with alcian blue. Alcian blue is a dye that forms complexes with... WebApr 6, 2024 · Alcian blue and safranin O staining were performed to verify the cartilage matrix deposition in the chondrocytes. As shown in Figure 3 A–C, the TE-Exo ADSCs group exhibited the strongest staining intensity and expressed increasing levels of GAGs compared to the control group.
Chondrocyte alcian blue staining
Did you know?
WebAlcian blue staining is used to determine sulfated proteoglycan distribution within the cell-seeded scaffolds after 28 days. Chondrocytes produced and were embedded a large amount of GAG-rich extracellular matrix, confirming the formation of cartilage lacuna structure ( Figure 5D ). WebJul 7, 2024 · The expressions of chondrocyte differentiation regulation genes, including Sox9, Ihh, CyclinD1, PTH1R, and hypertrophic chondrocyte marker genes including Col2α1, Runx2, MMP13, Col10α1 in the growth plate were significantly decreased in Runx1f/fCol2α1-cremice chondrocytes.
WebOct 10, 2024 · The slices were fixed on glass slides, deparaffined and then stained for the collagen and GAGs production analysis. In order to evaluate GAGs production, Alcian Blue acid mucins staining was done. WebJul 17, 2008 · (b) Staining of immature murine articular chondrocytes in primary cultures with Alcian blue, which reveals high production of sulfated proteoglycans in the extracellular matrix of chondrocytes ...
WebApr 21, 2024 · Alcian blue is a blue stain associated with the sulfated glycosaminoglycan of the cartilage matrix. After 16 days, the cells were rinsed once with PBS and fixed with 4% formaldehyde solution for 1 ... WebMar 25, 2024 · Alcian blue staining was used to assess proteoglycan accumulation as previously described. 26 Briefly, after 10 days of culture the agarose molds were removed from each well of the 96-well plate leaving behind the microtissues. Microtissues were washed in 0.2 ml de-ionized water and then stained in 0.2 ml of 1% HCL-alcian blue …
WebConsistently, in a directed in vitro chondrocyte differentiation assay, the Terc−/− cells failed in forming Collagen II expressing cells. These findings underscore the significance in maintaining proper telomere lengths in stem cells and their derivatives for regenerative medicine. ... Consistently, Alcian blue staining for Aggrecan was ... my android phone does not ringWebAlcian Blue stain analysis 1. After 14 days or longer under differentiating conditions, remove media from culture vessel, rinse once with DPBS, and fix cells with 4% formaldehyde solution for 30 minutes. 2. After fixation, rinse wells with DPBS and stain cells with 1% Alcian Blue solution prepared in 0.1 N HCL for 30 minutes. 3. my android phone is acting crazyWebMay 20, 2024 · ab150662 Alcian Blue, pH 2.5 (Mucin Stain) 5 5. Staining Protocol Equilibrate all materials and prepared reagents to room temperature just prior to … how to paint wood trimWebImmunohistochemical staining with Alcian blue is a convenient method to determine the extent of sulfation of mucins in tissues. For this purpose, the Alcian blue staining … how to paint wood stairsWebDec 13, 2024 · Deparaffinized sections were stained with Alcian Blue and van Gieson. The sections were scanned using a Nikon Eclipse 90i epi-fluorescence microscope equipped with a Nikon DS-Fi2 color head camera and a NIS-Elements imaging software suite (vD4.10.02; Nikon Instruments, Melville, NY, USA). how to paint wood to make it look weatheredWebApr 12, 2024 · Strikingly, staining for Alcian Blue and perilipin that are used for chondrocyte and adipocyte identification, respectively, were unaltered in mutant bone (Fig. 1G, H). how to paint wood stairs whiteWebIf you have your cells fixed prior to staining no problem. Also notice that the ph of the alcian blue may differ which GAG they will stain. pH 1.0 will stain sulphated mucins while ph2.5... my android phone is not ringing