Bodipy stain fixed cells
WebThese Lipi-probes are LD-specific probes that contain a pyrene or perylene group as a fluorescent scaffold and can be used to observe dynamics of LD in live cells and also interrelations with other organelles by simultaneous … WebInvitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction …
Bodipy stain fixed cells
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WebAug 1, 2024 · Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off. WebFixation of cells with paraformaldehyde is the method of choice, because the cells retain their lipid content and lipid droplet structure is unaffected. As more lipid droplet …
WebJun 8, 2015 · BODIPY works well for neutral lipid staining in live imaging. LipiTOX is for fixed cells. I tried LipiTOX for flow cytometry, the results weren't as good as BODIPY staining of droplets. WebWe routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final …
WebSep 5, 2016 · In this protocol, we describe the use of the fluorescent neutral lipid dye 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) to facilitate quantification of neutral lipid content by flow cytometry and observation of LDs by microscopy. Grant support P01 CA104838/CA/NCI NIH HHS/United States WebApr 11, 2024 · The cells on the coverslips were fixed with 4% paraformaldehyde for 20 min. After washing twice with PBS, the cells were treated with BODIPY 493/503 (1 μg/ml) for 20 min and DAPI (5 μg/ml) for 2 min. Images were acquired using a fluorescence microscope. Tumor growth and metastasis assay in nude mice
WebSep 5, 2016 · Fixed cells were stained with BODIPY 493/503 (ThermoFisher Scientific, Cleveland, OH, USA) at 10 lg/mL and Hoechst (ThermoFisher Scientific, Cleveland, OH, USA) at 1 lg/mL for 30 min at room...
WebNov 28, 2016 · Nile-Red and BODIPY (Invitrogen) staining was performed to measure the lipid levels in various breast cell lines. For lipid staining, 1 × 10 5 cells were seeded in a … ebb and flow animeWebCellTracker Invitrogen™ CM™-DiI (C7000, C7001) is a DiI derivative that is somewhat more water-soluble than DiIC 18 (3), thus facilitating the preparation of staining solutions for cell suspensions and fixed cells. In addition to its improved solubility in culture medium, CellTracker CM-DiI contains a thiol-reactive chloromethyl moiety that ... ebb and flow 5 gal bucket systemWebJun 28, 2024 · BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry Prepare 5 mM … ebb and flow activewearWebSep 25, 2024 · Here we describe a method to stain LDs, which can be applied to previously fixed mouse oocytes and embryos. This method is based on fluorescent dyes, Nile red … ebb and flow aquaponicsWebApr 7, 2024 · Safranin-O staining. Cells were fixed in 70% ethanol and incubated in 0.1% safranin-O for 5 min at room temperature and serially rinsed with double distilled water, 70% ethanol, and 100% ethanol. ... chondrogenic (C), and osteogenic (D) differentiation was assessed by oil Bodipy staining of neutral lipids, safranin-O staining of cartilage, and ... compassionate house stokeWebER-Tracker dyes are highly selective, cell-permeant, live cell endoplasmic reticulum stains. At low concentrations, these dyes have not been shown to be toxic to cells. When cells are stained using the protocol provided, the ER staining pattern is partially retained after fixation with formaldehyde. ebb and flo ebb and the new boatWebCan bodipy be fixed? We routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final concentration of BODIPY 493/503 depends on fat droplet size, the larger the fat droplets, the less stain is needed. How do you stain with bodipy? ebb and flow arena